{"id":3956,"date":"2021-12-17T16:27:52","date_gmt":"2021-12-17T14:27:52","guid":{"rendered":"https:\/\/ospt.osi.lv\/?p=3956"},"modified":"2022-01-11T12:16:52","modified_gmt":"2022-01-11T10:16:52","slug":"n-terminal-modification-of-gly-his-tagged-proteins-with-azidogluconolactone","status":"publish","type":"post","link":"https:\/\/ospt.osi.lv\/n-terminal-modification-of-gly-his-tagged-proteins-with-azidogluconolactone\/","title":{"rendered":"37. N-Terminal Modification of Gly-His-Tagged Proteins with Azidogluconolactone"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]Brune,K. D.; Liekni\u0146a, I.; Sutov, G.; Morris, A. R.; Jovicevic, D.; Kalni\u0146\u0161, G.; Kaz\u0101ks, A.; Kluga, R.; Kastaljana, S.; Zajakina, A.; Jansons, J.; Skrasti\u0146a, D.; Spunde, K.; Cohen, A. A.; Bjorkman, P. J.; Morris, H. R.; Suna, E.; T\u0101rs, K.<em>\u00a0ChemBioChem<\/em> <strong>2021<\/strong>,<em> 22, <\/em>3199-3207.<\/p>\n<p>DOI: <a title=\"N-Terminal Modification of Gly-His-Tagged Proteins with Azidogluconolactone\" href=\"https:\/\/doi.org\/10.1002\/cbic.202100381\" target=\"_blank\" rel=\"nofollow noopener\">10.1002\/cbic.202100381<\/a><\/p>\n<p><!--more--><\/p>\n<h4>Abstract<\/h4>\n<p style=\"text-align: justify;\"><b>Site-selective \u03b1-amino acylation<\/b> of proteins with N-terminal Gly-HisTags using 6-azido-6-deoxy-glucono-1,5-lactone (6AGDL) is reported. To demonstrate the usefulness of this reaction, we multimerise the SARS-CoV-2 receptor-binding domain (RBD) protein antigen onto virus-like particles (VLPs) via strain-promoted alkyne-azide cycloaddition (SPAAC). The resulting COVID-19 vaccine candidate induces single-shot seroconversion, and virus-neutralizing antibodies in mice.<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text]Brune,K. D.; Liekni\u0146a, I.; Sutov, G.; Morris, A. R.; Jovicevic, D.; Kalni\u0146\u0161, G.; Kaz\u0101ks, A.; Kluga, R.; Kastaljana, S.; Zajakina, A.; Jansons, J.; Skrasti\u0146a, D.; Spunde, K.; Cohen, A. A.; Bjorkman, P. J.; Morris, H. R.; Suna, E.; T\u0101rs, K.\u00a0ChemBioChem 2021, 22, 3199-3207. DOI: 10.1002\/cbic.202100381<\/p>\n","protected":false},"author":1,"featured_media":3957,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"image","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[16],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/posts\/3956"}],"collection":[{"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/comments?post=3956"}],"version-history":[{"count":2,"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/posts\/3956\/revisions"}],"predecessor-version":[{"id":4010,"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/posts\/3956\/revisions\/4010"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/media\/3957"}],"wp:attachment":[{"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/media?parent=3956"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/categories?post=3956"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ospt.osi.lv\/wp-json\/wp\/v2\/tags?post=3956"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}