Brune,K. D.; Liekniņa, I.; Sutov, G.; Morris, A. R.; Jovicevic, D.; Kalniņš, G.; Kazāks, A.; Kluga, R.; Kastaljana, S.; Zajakina, A.; Jansons, J.; Skrastiņa, D.; Spunde, K.; Cohen, A. A.; Bjorkman, P. J.; Morris, H. R.; Suna, E.; Tārs, K. ChemBioChem 2021, 22, 3199-3207.
Abstract
Site-selective α-amino acylation of proteins with N-terminal Gly-HisTags using 6-azido-6-deoxy-glucono-1,5-lactone (6AGDL) is reported. To demonstrate the usefulness of this reaction, we multimerise the SARS-CoV-2 receptor-binding domain (RBD) protein antigen onto virus-like particles (VLPs) via strain-promoted alkyne-azide cycloaddition (SPAAC). The resulting COVID-19 vaccine candidate induces single-shot seroconversion, and virus-neutralizing antibodies in mice.
Date: Dec 17, 2021
AUTHOR: Artis Kinens